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Nitrogen-15 (15 N) tracing is a technique to study the nitrogen cycle using the heavier, stable nitrogen isotope 15 N.Despite the different weights, 15 N is involved in the same chemical reactions as the more abundant 14 N and is therefore used to trace and quantify conversions of one nitrogen compound to another.
The lighter isotope of nitrogen, 14 N, is preferred during denitrification, leaving the heavier nitrogen isotope, 15 N, in the residual matter. This selectivity leads to the enrichment of 14 N in the biomass compared to 15 N. [27] Moreover, the relative abundance of 14 N can be analyzed to distinguish denitrification apart from other processes ...
Nitrogen cycle. Nitrification is the biological oxidation of ammonia to nitrate via the intermediary nitrite.Nitrification is an important step in the nitrogen cycle in soil.The process of complete nitrification may occur through separate organisms [1] or entirely within one organism, as in comammox bacteria.
The most common denitrification process is outlined below, with the nitrogen oxides being converted back to gaseous nitrogen: 2 NO 3 − + 10 e − + 12 H + → N 2 + 6 H 2 O. The result is one molecule of nitrogen and six molecules of water. Denitrifying bacteria are a part of the N cycle, and consists of sending the N back into the atmosphere.
Fixed nitrogen sources are required for most organisms to synthesize proteins, nucleic acids and other cellular components. Depending on the enzyme capabilities of the organism, nitrogen may be provided as bulk protein, such as soy meal; as pre-digested polypeptides, such as peptone or tryptone; or as ammonia or nitrate salts. Cost is also an ...
Industrial microbiology is a branch of biotechnology that applies microbial sciences to create industrial products in mass quantities, often using microbial cell factories. There are multiple ways to manipulate a microorganism in order to increase maximum product yields. Introduction of mutations into an organism may be accomplished by ...
The marine nitrogen cycle consists of complex microbial transformations which include the fixation of nitrogen, its assimilation, nitrification, anammox and denitrification. [78] Some of these processes take place in deep water so that where there is an upwelling of cold waters, and also near estuaries where land-sourced nutrients are present ...
15 N NMR is the most effective method for investigation of structure of heterocycles with a high content of nitrogen atoms (tetrazoles, triazines and their annelated analogs). [7] [8] 15 N labeling followed by analysis of 13 C– 15 N and 1 H– 15 N couplings may be used for establishing structures and chemical transformations of nitrogen ...