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The z-value is a measure of the change of the D-value with varying temperature, and is a simplified version of an Arrhenius equation and it is equivalent to z=2.303 RT T ref /E. [2] The z-value of an organism in a particular medium is the temperature change required for the D-value to change by a factor of ten, or put another way, the ...
The Z-factor defines a characteristic parameter of the capability of hit identification for each given assay. The following categorization of HTS assay quality by the value of the Z-Factor is a modification of Table 1 shown in Zhang et al. (1999); [2] note that the Z-factor cannot exceed one.
To find a negative value such as –0.83, one could use a cumulative table for negative z-values [3] which yield a probability of 0.20327. But since the normal distribution curve is symmetrical, probabilities for only positive values of Z are typically given.
A 0.5 McFarland standard is prepared by mixing 0.05 mL of 1.175% barium chloride dihydrate (BaCl 2 •2H 2 O), with 9.95 mL of 1% sulfuric acid (H 2 SO 4). [ 1 ] Now there are McFarland standards prepared from suspensions of latex particles, which lengthens the shelf life and stability of the suspensions.
The D-value at an unknown temperature can be calculated knowing the D-value at a given temperature provided the Z-value is known. The target of reduction in canning is the 12- D reduction of C. botulinum, which means that processing time will reduce the amount of this bacteria by a factor of 10 12 .
A bar chart is a graph that shows categorical data as bars presenting heights (vertical bar) or widths (horizontal bar) proportional to represent values. Bar charts provide an image that could also be represented in a tabular format. [10] In the bar chart example, we have the birth rate in Brazil for the December months from 2010 to 2016. [9]
This process was evaluated by functional assessments (cell density, enzymes activity, and ratio of the residual substrate) and compositional analysis. (2) Serial dilutions were conducted to the enriched effective microbial consortia. Six dilutions were prepared, from dilution 10 2 to 10 10 with 24 replicates. The dissimilarity between dilutions ...
The Miles and Misra Method (or surface viable count) is a technique used in Microbiology to determine the number of colony forming units in a bacterial suspension or homogenate. The technique was first described in 1938 by Miles, Misra and Irwin who at the time were working at the LSHTM. [1] The Miles and Misra method has been shown to be ...