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High-throughput phenotypic testing is increasingly important for exploring the biology of bacteria, fungi, yeasts, and animal cell lines such as human cancer cells.Just as DNA microarrays and proteomic technologies have made it possible to assay the expression level of thousands of genes or proteins all a once, phenotype microarrays (PMs) make it possible to quantitatively measure thousands of ...
High-throughput screening (HTS) is a method for scientific discovery especially used in drug discovery and relevant to the fields of biology, materials science [1] and chemistry. [ 2 ] [ 3 ] Using robotics , data processing/control software, liquid handling devices, and sensitive detectors, high-throughput screening allows a researcher to ...
Over recent years, many groups have successfully used genome-wide CRISPR/Cas9 as a screening strategy for HDFs in viral infections. [ 59 ] One example is provided by Marceau et al. (2017), [ 60 ] who aimed to dissect the host factors associated with dengue and hepatitis C (HCV) infection (two viruses in family Flaviviridae ).
High-content screening where changes in the expression of several proteins can be simultaneously monitored is also often used. [9] [10] High-content imaging of dye-labeled cellular components can also reveal effects of compounds on cell cultures in vitro, distinguishing the phenotypic effects of a broad variety of drugs. [11]
The Cell Painting assay [1] is a high-content, high-throughput imaging technique used to capture a wide array of cellular phenotypes in response to diverse perturbations. [2] These phenotypes, often termed "morphological profiles", can be used to understand various biological phenomena, including cellular responses to genetic changes, drug ...
The choice of a chemical library is less stringent than other high-throughput screening strategies owing to the lack of functional readouts, which would otherwise require deconvolution of the source compound that generates biological activity. Thus, the typical range for AS-MS is 400-3,000 compounds per pool. [20]
Freshman Jeremiah Fears scored a season-high 26 points, Jalon Moore added 24 and Oklahoma held off No. Arizona 82-77 on Thursday in the semifinals of the Battle 4 Atlantis. Duke Miles made a high ...
Advanced development of 3D models for high-throughput screening in high density formats has recently been achievable due to technological achievements related to increased microplate density. These can be found in 384 and 1536-well formats that are cell repellent, cost effective, and amenable to fully automated screening platforms. [ 69 ]