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Ammonium sulfate is a precursor to other ammonium salts, especially ammonium persulfate. Ammonium sulfate is listed as an ingredient for many United States vaccines per the Centers for Disease Control. [12] Ammonium sulfate has also been used in flame retardant compositions acting much like diammonium phosphate.
Ammonium sulfate is an inorganic salt with a high solubility that disassociates into ammonium (NH + 4) and sulfate (SO 2− 4) in aqueous solutions. [1] Ammonium sulfate is especially useful as a precipitant because it is highly soluble, stabilizes protein structure, has a relatively low density, is readily available, and is relatively inexpensive.
Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out) [1] is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength.
The most commonly used salt is ammonium sulfate. There is a low variation in salting out over temperatures 0 °C to 30 °C. Protein precipitates left in the salt solution can remain stable for years-protected from proteolysis and bacterial contamination by the high salt concentrations.
The following chart shows the solubility of various ionic compounds in water at 1 atm pressure and room temperature (approx. 25 °C, 298.15 K). "Soluble" means the ionic compound doesn't precipitate, while "slightly soluble" and "insoluble" mean that a solid will precipitate; "slightly soluble" compounds like calcium sulfate may require heat to precipitate.
Substance Formula 0 °C 10 °C 20 °C 30 °C 40 °C 50 °C 60 °C 70 °C 80 °C 90 °C 100 °C Barium acetate: Ba(C 2 H 3 O 2) 2: 58.8: 62: 72: 75: 78.5: 77: 75
Urea- Ammonium sulfate solution 28-32% 63.0 Diammonium phosphate 18% ... High osmotic pressure is when there is a higher concentration of salts inside the root cell ...
dideuterated ammonium chromium sulfate hexahydrate bright blue, formed from with ammonium sulfate in minimal water under nitrogen gas. Stable in air from oxidation, but may dehydrate. [24] K Cu K 2 [Cu(H 2 O) 6](SO 4) 2: cyanochroite [14] 9.27 12.44 6.30 104.47 [25] 663.0 [25]