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The Kastle–Meyer test is a presumptive blood test, first described in 1903, in which the chemical indicator phenolphthalein is used to detect the possible presence of hemoglobin. It relies on the peroxidase -like activity of hemoglobin in blood to catalyze the oxidation of phenolphthalin (the colorless reduced form of phenolphthalein) into ...
Kastle-Meyer test for blood; Malachite green test for blood; Marquis reagent for narcotics and alkaloids; Benzidine test for blood which changes from clear to blue in color with the presence of blood; Luminol, phenolphthalein, Hemastix, Hemident, and Bluestar are all also used for blood testing [citation needed]
As with all presumptive tests, this technique can produce false positive results due to metals and strong chemicals, such as bleach, that will also react. Another common presumptive test is the Kastle-Meyer or Phenolphthalein test. This is a catalytic test that detects the heme group in blood that transports oxygen and carbon dioxide. [5]
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A reduced form of phenolphthalein, phenolphthalin, which is colorless, is used in a test to identify substances thought to contain blood, commonly known as the Kastle–Meyer test. A dry sample is collected with a swab or filter paper.
The Kastle Meyer (KM) reagent is actually already basic in pH -- it is when the reduced phenolphthalein in the reagent is oxidized to phenolphthalein by the presence of heme (or some other oxidizer) that it is the phenolphthalein you are familiar with, which indeed, will turn pink in the presence of its alkaline solvent.
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Kastle-Meyer test; Kastle Systems, an American security firm This page was last edited on 23 June 2023, at 16:54 (UTC). Text is available under the Creative ...