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  2. Lambda phage - Wikipedia

    en.wikipedia.org/wiki/Lambda_phage

    Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). It was discovered by Esther Lederberg in 1950. [ 2 ]

  3. Integration host factor - Wikipedia

    en.wikipedia.org/wiki/Integration_host_factor

    HU and integration host factor function as auxiliary proteins in cleavage of phage lambda cohesive ends by terminase is an academic journal written by the Department of Molecular Genetics. In their article, they created isogenic strains of E.coli that were lacking HU or integration host factors to test whether bacteriophage would grow under ...

  4. Sticky and blunt ends - Wikipedia

    en.wikipedia.org/wiki/Sticky_and_blunt_ends

    The simplest DNA end of a double stranded molecule is called a blunt end. Blunt ends are also known as non-cohesive ends. In a blunt-ended molecule, both strands terminate in a base pair. Blunt ends are not always desired in biotechnology since when using a DNA ligase to join two molecules into one, the yield is significantly lower with blunt ...

  5. BamHI - Wikipedia

    en.wikipedia.org/wiki/BamHI

    BamHI, like other type II restriction endonucleases, often requires divalent metals as cofactors to catalyze DNA cleavage. [2] Two-metal ion mechanism is one of the possible catalytic mechanisms of BamHI since the BamHI crystal structure has the ability to bind two metal ions at the active site, which is suitable for the classical two-metal ion mechanism to proceed.

  6. PstI - Wikipedia

    en.wikipedia.org/wiki/PstI

    PstI cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. [1] This cleavage yields sticky ends 4 base pairs long. PstI is catalytically active as a dimer.

  7. EcoRI - Wikipedia

    en.wikipedia.org/wiki/EcoRI

    EcoRI (pronounced "eco R one") is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system. [1]

  8. Joey Galloway: Indiana should bench QB Kurtis Rourke vs ... - AOL

    www.aol.com/joey-galloway-indiana-bench-qb...

    ESPN's Joey Galloway on Tuesday said Indiana football shouldn't play quarterback Kurtis Rourke vs. Ohio State to keep its CFP path intact.

  9. Fixed-point combinator - Wikipedia

    en.wikipedia.org/wiki/Fixed-point_combinator

    A function's identity is based on its implementation. A lambda calculus function (or term) is an implementation of a mathematical function. In the lambda calculus there are a number of combinators (implementations) that satisfy the mathematical definition of a fixed-point combinator.