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The genome organisation of HBV; the genes overlap. ORF S, in green, encodes HBsAg. HBsAg under a transmission electron microscope: the protein self assembles into virus-like particles. HBsAg (also known as the Australia antigen) is the surface antigen of the hepatitis B virus (HBV). Its presence in blood indicates existing hepatitis B infection.
In quantitative ELISA, the optical density (OD) of the sample is compared to a standard curve, which is typically a serial dilution of a known-concentration solution of the target molecule. For example, if a test sample returns an OD of 1.0, the point on the standard curve that gave OD = 1.0 must be of the same analyte concentration as the sample.
HBcAg is an antigen that can be found on the surface of the nucleocapsid core (the inner most layer of the hepatitis B virus).While both HBcAg and HBeAg are made from the same open reading frame, HBcAg is not secreted as a monomer. [3]
Qualitative assays, i.e. assays which generally give just a pass or fail, or positive or negative or some such sort of only small number of qualitative gradation rather than an exact quantity. Semi-quantitative assays , i.e. assays that give the read-out in an approximate fashion rather than an exact number for the quantity of the substance.
A newer approach to immunoassays involves combining real-time quantitative polymerase chain reaction (RT qPCR) and traditional immunoassay techniques. Called real-time immunoquantitative PCR (iqPCR) the label used in these assays is a DNA probe.
The general expression Qualitative Analysis [...] refers to analyses in which substances are identified or classified on the basis of their chemical or physical properties, such as chemical reactivity, solubility, molecular weight, melting point, radioactivity properties (emission, absorption), mass spectra, nuclear half-life, etc. Quantitative Analysis refers to analyses in which the amount ...
Lacy also opted out of playing in the Texas Bowl versus Baylor. Lacy played three seasons at LSU after transferring from Louisiana. He compiled 112 receptions for 1,692 yards and 16 touchdowns in ...
That this perspective tends to dominate the evidence-based practice literature makes the merit of qualitative research unclear;" 1 Some people view qualitative research as less beneficial and effective, with its lack of numbers, the fact that it is "feeling-based" research, makes the opponents associate it with bias. Nevertheless, the ability ...