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The ferric chloride test is used to determine the presence of phenols in a given sample or compound (for instance natural phenols in a plant extract). Enols , hydroxamic acids , oximes, and sulfinic acids give positive results as well. [ 1 ]
The affinity of iron(III) for oxygen ligands was the basis of qualitative tests for phenols. Although superseded by spectroscopic methods, the ferric chloride test is a traditional colorimetric test. [26] The affinity of iron(III) for phenols is exploited in the Trinder spot test. [27]
The following describes the use of ferric chloride (FeCl3) tests for phenolics in general: Powdered plant leaves of the test plant (1.0 g) are weighed into a beaker and 10 ml of distilled water are added. The mixture is boiled for five minutes.
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A few types of aromatic compounds, such as phenol, will react without a catalyst, but for typical benzene derivatives with less reactive substrates, a Lewis acid is required as a catalyst. Typical Lewis acid catalysts include AlCl 3, FeCl 3, FeBr 3 and ZnCl 2. These work by forming a highly electrophilic complex which is attacked by the benzene ...
The Folin–Ciocâlteu reagent (FCR) or Folin's phenol reagent or Folin–Denis reagent, is a mixture of phosphomolybdate and phosphotungstate used for the colorimetric in vitro assay of phenolic and polyphenolic antioxidants, also called the gallic acid equivalence method (GAE). [1] It is named after Otto Folin, Vintilă Ciocâlteu, and Willey ...