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Basic fuchsine is a mixture of rosaniline, pararosaniline, new fuchsine and magenta II [Wikidata]. [15] Formulations usable for making of Schiff reagent must have high content of pararosanilin. The actual composition of basic fuchsine tends to somewhat vary by vendor and batch, making the batches differently suitable for different purposes.
Fuchsine: Basic fuchsin Rosalinin Magenta I Basic violet 14 42510 triarylmethane 632-99-5: Gallamin blue Mordant blue 45 51045 oxazin 1563-02-6: Gallocyanin: Mordant blue 10 51030 oxazin 1562-85-2: Gossypetin: 75750 natural 489-35-0: Green S: Acid green 50 Food green 4 44090 triarylmethane 3087-16-9: Guinea green B Guinea green Acid green 3 ...
Carbol fuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint (CAS) is a mixture of phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for the mycolic acids found in their cell membranes .
Pararosaniline, pararosaniline free base, Basic Red 9, or C.I. 42500 is an organic compound with the formula (H 2 NC 6 H 4) 2 C=(C 6 H 4 NH). [1] It is the free base form of pararosaniline hydrochloride , [(H 2 NC 6 H 4 ) 3 C] + Cl − , a magenta solid with a variety of uses as a dye .
The Kinyoun method can be modified as a weak acid fast stain, which uses 0.5–1.0% sulfuric acid instead of hydrochloric acid.The weak acid fast stain, in addition to staining Mycobacteria, will also stain organisms that are not able to maintain the carbol fuchsin after decolorizing with HCl, such as Nocardia species and Cryptosporidium.
The structure of the fuchsin dye. The Schiff test is an early organic chemistry named reaction developed by Hugo Schiff, [1] and is a relatively general chemical test for detection of many organic aldehydes that has also found use in the staining of biological tissues. [2]
New fuchsine is an organic compound with the formula [(H 2 N(CH 3)C 6 H 3) 3 C]Cl. It is a green-colored solid that is used as a dye of the triarylmethane class. It is one of the four components of basic fuchsine , and one of the two that are available as single dyes. [ 1 ]
After the Ziehl-Neelsen staining procedure using carbol fuchsin, acid-fast bacteria are observable as vivid red or pink rods set against a blue or green background, depending on the specific counterstain used, such as methylene blue or malachite green, respectively. Non-acid-fast bacteria and other cellular structures will be colored by the ...