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Also common in the microscopy literature is a formula for resolution that treats the above-mentioned concerns about contrast differently. [2] The resolution predicted by this formula is proportional to the Rayleigh-based formula, differing by about 20%. For estimating theoretical resolution, it may be adequate.
The spectral resolution of a spectrograph, or, more generally, of a frequency spectrum, is a measure of its ability to resolve features in the electromagnetic spectrum.It is usually denoted by , and is closely related to the resolving power of the spectrograph, defined as =, where is the smallest difference in wavelengths that can be distinguished at a wavelength of .
IUPAC definition. The IUPAC definition for resolution in mass spectrometry is. Where a larger resolution indicates a better separation of peaks. [1][2] This definition is used in a number of mass spectrometry texts. [3][4][5][6][7][8][9][10][11] This use is also implied by the term "high-resolution mass spectrometry." [12]
Resolution (chromatography) Example chromatogram showing signal as a function of retention time. In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. [1][2][3][4]
The fundamental resolution equation is used in chromatography to help relate adjustable chromatographic parameters to resolution, and is as follows: R s = [N 1/2 /4] [ (α-1)/α] [k 2 '/ (1+k 2 ')], where. N = Number of theoretical plates. α = Selectivity Term = k 2 '/k 1 '. The [N 1/2 /4] term is the column factor, the [ (α-1)/α] term is ...
Fourier-transform infrared spectroscopy (FTIR) [1] is a technique used to obtain an infrared spectrum of absorption or emission of a solid, liquid, or gas. An FTIR spectrometer simultaneously collects high-resolution spectral data over a wide spectral range. This confers a significant advantage over a dispersive spectrometer, which measures ...
However, the acid diffusion length is itself a potential resolution limiter. [18] In addition, too much diffusion reduces chemical contrast, leading again to more roughness. [17] The following reactions are an example of commercial chemically amplified photoresists in use today: photoacid generator + hν (193 nm) → acid cation + sulfonate ...
Resolution in the context of structural biology is the ability to distinguish the presence or absence of atoms or groups of atoms in a biomolecular structure. Usually, the structure originates from methods such as X-ray crystallography, electron crystallography, or cryo-electron microscopy. The resolution is measured of the "map" of the ...