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MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES, [1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring.
A buffer solution is a solution where the pH does not change significantly on dilution or if an acid or base is added at constant temperature. [1] Its pH changes very little when a small amount of strong acid or base is added to it. Buffer solutions are used as a means of keeping pH at a nearly constant value in a wide variety of chemical ...
MOPSO has been used as a buffer component for: [4] Copper analysis via: Flow injection micellar technique of the catalytic reaction of 3-methyl-2-benzothiazolinone hydrazone and N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline; Electrospray ionization quadrupole time-of-flight mass spectroscopy to measure MOPSO-copper chelated complexes
The effect of buffer identity on the kinetics of the restriction enzyme EcoRV has been studied in various buffers, including Bis-Tris propane. [5] Bis-Tris propane wide buffering range is also useful for calibration of genetically encoded pH indicators expressed in the cytosol or mitochondria. [ 6 ]
Total ionic strength adjustment buffer (TISAB) is a buffer solution which increases the ionic strength of a solution to a relatively high level. This is important for potentiometric measurements, including ion selective electrodes , because they measure the activity of the analyte rather than its concentration.
McIlvaine buffer is a buffer solution composed of citric acid and disodium hydrogen phosphate, also known as citrate-phosphate buffer. It was introduced in 1921 by the United States agronomist Theodore Clinton McIlvaine (1875–1959) from West Virginia University , and it can be prepared in pH 2.2 to 8 by mixing two stock solutions.
Sigma-Aldrich (formally MilliporeSigma) [5] [6] is an American chemical, life science, and biotechnology company owned by the multinational chemical conglomerate Merck Group Sigma-Aldrich was created in 1975 by the merger of Sigma Chemical Company and Aldrich Chemical Company.
This reaction is rapid and stoichiometric, with the addition of one mole of thiol releasing one mole of TNB. The TNB 2− is quantified in a spectrophotometer by measuring the absorbance of visible light at 412 nm, using an extinction coefficient of 14,150 M −1 cm −1 for dilute buffer solutions, [4] [5] and a coefficient of 13,700 M −1 cm −1 for high salt concentrations, such as 6 M ...