Search results
Results from the WOW.Com Content Network
Radiolabeling or radiotracing is thus the radioactive form of isotopic labeling. In biological contexts, experiments that use radioisotope tracers are sometimes called radioisotope feeding experiments. Radioisotopes of hydrogen, carbon, phosphorus, sulfur, and iodine have been used extensively to trace the path of biochemical reactions.
Radiolabeling is not necessary for some applications. For some purposes, soluble ionic salts can be used directly without further modification (e.g., gallium-67, gallium-68, and radioiodine isotopes). These uses rely on the chemical and biological properties of the radioisotope itself, to localize it within the organism or biological system.
Isotopic labeling (or isotopic labelling) is a technique used to track the passage of an isotope (an atom with a detectable variation in neutron count) through chemical reaction, metabolic pathway, or a biological cell. [1]
The harsh conditions needed for the addition of the into the biomacromolecule can easily hinder its use in radiolabeling reactions. [1] In order to overcome these obstacles, protein or peptide labeling can be performed through a prosthetic group or bifunctional labeling agent to which the radiofluorine has been attached.
Positron emission tomography (PET) [1] is a functional imaging technique that uses radioactive substances known as radiotracers to visualize and measure changes in metabolic processes, and in other physiological activities including blood flow, regional chemical composition, and absorption.
A radioligand is a microscopic particle which consists of a therapeutic radioactive isotope and the cell-targeting compound - the ligand. The ligand is the target binding site, it may be on the surface of the targeted cancer cell for therapeutic purposes.
Before the advent of radiolabeling, this was the only group of techniques which could infer function based on biochemical activity and it represented a great promise not only for basic science, such as physiology and pharmacology, but for pathology and laboratory diagnosis of diseases, as well.
Frank James Dixon (March 9, [1] 1920 – February 8, 2008) was an American biomedical researcher, known for his research into autoimmunity – diseases of the immune system that can damage other organs of the body – as well as for developing radioactive labeling techniques to study proteins. [2]