Search results
Results from the WOW.Com Content Network
An autoclave is a machine used to carry out industrial and scientific processes requiring elevated temperature and pressure in relation to ambient pressure and/or temperature. Autoclaves are used before surgical procedures to perform sterilization and in the chemical industry to cure coatings and vulcanize rubber and for hydrothermal synthesis .
An extraordinary heat-tolerant hyperthermophile is Geogemma barossii (Strain 121), [5] which has been able to double its population during 24 hours in an autoclave at 121 °C (hence its name). The current record growth temperature is 122 °C, for Methanopyrus kandleri.
[citation needed] If autoclaving is not possible because of lack of equipment, or the need to sterilize something that will not withstand the higher temperature, unpressurized heating for a prolonged period at a temperature of up to 100 °C (212 °F), the boiling point of water, may be used.
Proper autoclave treatment will inactivate all resistant bacterial spores in addition to fungi, bacteria, and viruses, but is not expected to eliminate all prions, which vary in their heat resistance. For prion elimination, various recommendations state 121–132 °C (250–270 °F) for 60 minutes or 134 °C (273 °F) for at least 18 minutes. [14]
Pressure is then applied so that the interior temperature reaches 121 °C (250 °F), and this temperature is maintained for between 15 and 30 minutes. This elevated temperature and pressure is sufficient to sterilize samples of any commonly encountered microbes or spores.
Autoclave tape is an adhesive tape used in autoclaving (heating under high pressure with steam to sterilise) to indicate whether a specific temperature has been reached. [1] Autoclave tape works by changing color after exposure to temperatures commonly used in sterilization processes, typically 121°C in a steam autoclave.
The z-value is a measure of the change of the D-value with varying temperature, and is a simplified version of an Arrhenius equation and it is equivalent to z=2.303 RT T ref /E. [2] The z-value of an organism in a particular medium is the temperature change required for the D-value to change by a factor of ten, or put another way, the ...
An autoclave (≥ 121 °C) is a type of pressure cooker used by laboratories and hospitals to sterilize equipment. A stovetop autoclave is essentially a higher-pressure cooker with a gauge, used as an autoclave in poorer areas. Pressure canners are large pressure cookers which have the capacity to hold jars used in home canning.