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The procedure for seed plants describe above, fusion of moss protoplasts can be initiated without electric shock but by the use of polyethylene glycol (PEG). Further, moss protoplasts do not need phytohormones for regeneration, and they do not form a callus. [5] Instead, regenerating moss protoplasts behave like germinating moss spores. [6]
Protoplast (from Ancient Greek πρωτόπλαστος (prōtóplastos) 'first-formed'), is a biological term coined by Hanstein in 1880 to refer to the entire cell, excluding the cell wall. [ 1 ] [ 2 ] Protoplasts can be generated by stripping the cell wall from plant , [ 3 ] bacterial , [ 4 ] [ 5 ] or fungal cells [ 5 ] [ 6 ] by mechanical ...
Protoplast fusion is a technique in which transformed bacterial cells are treated with lysozyme in order to remove the cell wall. Following this, fusogenic agents (e.g., Sendai virus, PEG, electroporation) are used in order to fuse the protoplast carrying the gene of interest with the target recipient cell.
Hybrids may also be produced by a technique called protoplast fusion. In this case protoplasts are fused, usually in an electric field. Viable recombinants can be regenerated in culture. Chemical mutagens like ethyl methanesulfonate (EMS) and dimethyl sulfate (DMS), radiation, and transposons are used for mutagenesis. Mutagenesis is the ...
Protoplast fusion is the joining of cells or cell components to transfer traits between species. For example, the trait of male sterility is transferred from radishes to red cabbages by protoplast fusion. This male sterility helps plant breeders make hybrid crops. [4]
He also encouraged others to have a spinal fusion surgery in July 2023 that he claimed allowed him to walk pain-free without medications. “The surgery wasn’t nearly as scary as I made it out ...
Electroporation has also been used as a mechanism to trigger cell fusion. Artificially induced cell fusion can be used to investigate and treat different diseases, like diabetes, [10] [11] [12] regenerate axons of the central nerve system, [13] and produce cells with desired properties, such as in cell vaccines for cancer immunotherapy. [14]
Baby Has $5 Million Surgery to Remove Left Side of Brain at Just 4 Weeks Old. Now He's Waiting for the Next One (Exclusive) Hannah Sacks. December 17, 2024 at 1:00 AM.