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Used to examine objects that are too small to be seen by the naked eye: Cell biology: Molecular cloning: Used to assemble recombinant DNA molecules and to direct their replication within host organisms. [12] Molecular biology: Northern blot: Used to study gene expression by detection of RNA (or isolated mRNA) in a sample. [13] [14] Molecular ...
Cell biology (also cellular biology or cytology) is a branch of biology that studies the structure, function, and behavior of cells. [1] [2] All living organisms are made of cells. A cell is the basic unit of life that is responsible for the living and functioning of organisms. [3] Cell biology is the study of the structural and functional ...
Used for transcriptomics experiments on single cells. RSEM [127] 2011 2017 High, multi-thread, low RAM requirement Can estimate frequency of alternatively spliced transcripts. User friendly. StringTie [98] [128] 2015 2019 High, multi-thread, low RAM requirement Can use a combination of reference-guided and de novo assembly methods to identify ...
Thus, in its broader sense, "tissue culture" is often used interchangeably with "cell culture". On the other hand, the strict meaning of "tissue culture" refers to the culturing of tissue pieces, i.e. explant culture. Tissue culture is an important tool for the study of the biology of cells from multicellular organisms.
Outline of cell biology. Cell structure: Cell coined by Robert Hooke; Techniques: cell culture – flow cytometry – microscope – light microscope – electron microscopy – SEM – TEM – live cell imaging; Organelles: Cytoplasm – Vacuole – Peroxisome – Plastid. Cell nucleus. Nucleoplasm – Nucleolus – Chromatin – Chromosome ...
A metaphase cell positive for the BCR/ABL rearrangement using FISH. Cytogenetics is essentially a branch of genetics, but is also a part of cell biology/cytology (a subdivision of human anatomy), that is concerned with how the chromosomes relate to cell behaviour, particularly to their behaviour during mitosis and meiosis. [1]
This single cell shows the process of the central dogma of molecular biology, which are all steps researchers are interested to quantify (DNA, RNA, and Protein).. In cell biology, single-cell analysis and subcellular analysis [1] refer to the study of genomics, transcriptomics, proteomics, metabolomics, and cell–cell interactions at the level of an individual cell, as opposed to more ...
Methods of cell sorting fall into two major categories: fluorescence-activated cell sorting (FACS) and immunomagnetic cell sorting. [2] Due to many years of refinement and increased demand for cell separation however, researchers are working to develop microfluidic sorting devices that have many benefits in comparison to the main types of fluorescence-activated cell sorting and immunomagnetic ...
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