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Hybridization is a basic property of nucleotide sequences and is taken advantage of in numerous molecular biology techniques. Overall, genetic relatedness of two species can be determined by hybridizing segments of their DNA (DNA-DNA hybridization). Due to sequence similarity between closely related organisms, higher temperatures are required ...
In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
In genomics, DNA–DNA hybridization is a molecular biology technique that measures the degree of genetic similarity between DNA sequences. It is used to determine the genetic distance between two organisms and has been used extensively in phylogeny and taxonomy .
Fluorescence in situ hybridization (FISH) is a molecular cytogenetic technique that uses fluorescent probes that bind to only particular parts of a nucleic acid sequence with a high degree of sequence complementarity.
Hybridization (or hybridisation) may refer to: Hybridization (biology) , the process of combining different varieties of organisms to create a hybrid Orbital hybridization , in chemistry, the mixing of atomic orbitals into new hybrid orbitals
Molecular biology techniques are common methods used in molecular biology, biochemistry, genetics and biophysics which generally involve manipulation and analysis of DNA, RNA, protein, and lipid Wikimedia Commons has media related to Molecular biology techniques .
In the hybridization-ligation assay [4] [5] a template probe replaces the capture probe in the sandwich assay for immobilization to the solid support. The template probe is fully complementary to the oligonucleotide analyte and is intended to serve as a substrate for T4 DNA ligase-mediated ligation.
In molecular biology, a hybridization probe (HP) is a fragment of DNA or RNA, usually 15–10000 nucleotides long, which can be radioactively or fluorescently labeled.HPs can be used to detect the presence of nucleotide sequences in analyzed RNA or DNA that are complementary to the sequence in the probe. [1]