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Optical activity is reciprocal, i.e. it is the same for opposite directions of wave propagation through an optically active medium, for example, clockwise polarization rotation from the point of view of an observer. In case of optically active isotropic media, the rotation is the same for any direction of wave propagation.
For an optically active substance, defined by [α] θ λ = α/γl, where α is the angle through which plane polarized light is rotated by a solution of mass concentration γ and path length l. Here θ is the Celsius temperature and λ the wavelength of the light at which the measurement is carried out. [2]
Linearly polarized light 5. Sample tube containing chiral molecules under study 6. Optical rotation due to molecules 7. Rotatable linear analyzer 8. Detector. A polarimeter [1] is a scientific instrument used to measure optical rotation: the angle of rotation caused by passing linearly polarized light through an optically active substance. [2]
Optically active samples, such as solutions of chiral molecules, often exhibit circular birefringence. Circular birefringence causes rotation of the polarization of plane polarized light as it passes through the sample. In ordinary light, the vibrations occur in all planes perpendicular to the direction of propagation.
For wavelengths that are absorbed by the optically active sample, the two circularly polarized components will be absorbed to differing extents. This unequal absorption is known as circular dichroism. Circular dichroism causes incident linearly polarized light to become elliptically polarized. The two phenomena are closely related, just as are ...
Polarized light with its electric field along the plane of incidence is thus denoted p-polarized, while light whose electric field is normal to the plane of incidence is called s-polarized. P-polarization is commonly referred to as transverse-magnetic (TM), and has also been termed pi-polarized or π-polarized, or tangential plane polarized.
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