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The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
Inoculation of a TSI slant shows an alkaline slant and acidic, but with no gas, or H 2 S production. Following incubation on SIM, the culture appears nonmotile with no H 2 S production. Addition of Kovac's reagent to the SIM tube following growth typically indicates no indole formation (serotypes 2, 7, and 8 produce indole [5]).
A TSI slant is often used to distinguish nonfermenting Pseudomonas species from enteric pathogens in faecal specimens. [citation needed] When P. aeruginosa is isolated from a normally sterile site (blood, bone, deep collections), it is generally considered dangerous, and almost always requires treatment.
Simmons’ agar can be bought from suppliers as ready-made powders or slants. A slant is prepared by adding the heated agar to a test tube and allowing it to solidify at a slanted angle. To transfer cells from a sample to the agar, a sterilized needle is used to select a distinct colony from the sample and to streak across the agar surface, as ...
Salmonella growing on XLD agar. Xylose lysine deoxycholate agar (XLD agar) is a selective growth medium used in the isolation of Salmonella and Shigella species from clinical samples and from food.
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Hektoen enteric agar (HEK, HE or HEA) is a selective and differential agar [1] primarily used to recover Salmonella and Shigella from patient specimens. HEA contains indicators of lactose fermentation and hydrogen sulfide production; as well as inhibitors to prevent the growth of Gram-positive bacteria.