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  2. Immunostaining - Wikipedia

    en.wikipedia.org/wiki/Immunostaining

    A flow cytometer can be used for the direct analysis of cells expressing one or more specific proteins. Cells are immunostained in solution using methods similar to those used for immunofluorescence, and then analysed by flow cytometry. [citation needed]

  3. Flow cytometry - Wikipedia

    en.wikipedia.org/wiki/Flow_cytometry

    The main advantages of flow cytometry over histology and IHC is the possibility to precisely measure the quantities of antigens and the possibility to stain each cell with multiple antibodies-fluorophores, in current laboratories around 10 antibodies can be bound to each cell.

  4. Tetramer assay - Wikipedia

    en.wikipedia.org/wiki/Tetramer_assay

    The tetramers are labeled with a fluorophore, allowing tetramer-bound T-cells to be analyzed with flow cytometry. [4] Quantification and sorting of T-cells by flow cytometry enables researchers to investigate immune response to viral infection and vaccine administration as well as functionality of antigen-specific T-cells. [5]

  5. Immunohistochemistry - Wikipedia

    en.wikipedia.org/wiki/Immunohistochemistry

    Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.

  6. CyTOF - Wikipedia

    en.wikipedia.org/wiki/CyTOF

    Four to five polymers are bound to an antibody, resulting in about 100 isotope atoms per antibody. [14] Tagged antibodies may be in solution, conjugated to beads, or surface immobilized. The cell staining follows the same procedures as in fluorescent staining for flow cytometry. [14]

  7. Immunophenotyping - Wikipedia

    en.wikipedia.org/wiki/Immunophenotyping

    Immunophenotyping is a very common flow cytometry test in which fluorophore-conjugated antibodies are used as probes for staining target cells with high avidity and affinity. This technique allows rapid and easy phenotyping of each cell in a heterogeneous sample according to the presence or absence of a protein combination. [1]

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