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  2. Beer–Lambert law - Wikipedia

    en.wikipedia.org/wiki/Beer–Lambert_law

    Absorbance within range of 0.2 to 0.5 is ideal to maintain linearity in the Beer–Lambert law. If the radiation is especially intense, nonlinear optical processes can also cause variances. The main reason, however, is that the concentration dependence is in general non-linear and Beer's law is valid only under certain conditions as shown by ...

  3. Molar absorption coefficient - Wikipedia

    en.wikipedia.org/wiki/Molar_absorption_coefficient

    The absorbance of a material that has only one absorbing species also depends on the pathlength and the concentration of the species, according to the Beer–Lambert law =, where ε is the molar absorption coefficient of that material; c is the molar concentration of those species; ℓ is the path length.

  4. Variable pathlength cell - Wikipedia

    en.wikipedia.org/wiki/Variable_pathlength_cell

    Variable pathlength absorption spectroscopy uses a determined slope to calculate concentration. As stated above this is a product of the molar absorptivity and the concentration. Since the actual absorbance value is taken at many data points at equal intervals, background subtraction is generally unnecessary.

  5. Bradford protein assay - Wikipedia

    en.wikipedia.org/wiki/Bradford_protein_assay

    The equation displayed on the chart gives a means for calculating the absorbance and therefore concentration of the unknown samples. In Graph 1, x is concentration and y is absorbance, so one must rearrange the equation to solve for x and enter the absorbance of the measured unknown. [ 25 ]

  6. Nucleic acid quantitation - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_quantitation

    To normalize the concentration to a 10mm equivalent, the following is done: 0.6 OD X (10/3) * 50 μg/mL=100 μg/mL Most spectrophotometers allow selection of the nucleic acid type and path length such that resultant concentration is normalized to the 10 mm path length which is based on the principles of Beer's law.

  7. Atomic absorption spectroscopy - Wikipedia

    en.wikipedia.org/wiki/Atomic_absorption_spectroscopy

    The technique makes use of the atomic absorption spectrum of a sample in order to assess the concentration of specific analytes within it. It requires standards with known analyte content to establish the relation between the measured absorbance and the analyte concentration and relies therefore on the Beer–Lambert law.

  8. Benesi–Hildebrand method - Wikipedia

    en.wikipedia.org/wiki/Benesi–Hildebrand_method

    Concerns have also surfaced over the accuracy of the Benesi–Hildebrand method as certain conditions cause these calculations to become invalid. For instance, the reactant concentrations must always obey the assumption that the initial concentration of the guest ([G] 0) is much larger than the initial concentration of the host ([H] 0).

  9. Molar concentration - Wikipedia

    en.wikipedia.org/wiki/Molar_concentration

    Molar concentration or molarity is most commonly expressed in units of moles of solute per litre of solution. [2] For use in broader applications, it is defined as amount of substance of solute per unit volume of solution, or per unit volume available to the species, represented by lowercase : [3]