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2. Oil immersion - Wikipedia

   en.wikipedia.org/wiki/Oil_immersion

   Oil-immersion objective in use. From the above it is understood that oil between the specimen and the objective lens improves the resolving power by a factor 1/n. Objectives specifically designed for this purpose are known as oil immersion objectives. Oil immersion objectives are used only at very large magnifications that require high ...

3. Index-matching material - Wikipedia

   en.wikipedia.org/wiki/Index-matching_material

   Immersion oils are transparent oils that have specific optical and viscosity characteristics necessary for use in microscopy. Typical oils used have an index of refraction around 1.515. [ 2 ] An oil immersion objective is an objective lens specially designed to be used in this way.

4. Dark-field microscopy - Wikipedia

   en.wikipedia.org/wiki/Dark-field_microscopy

   In optical microscopes a darkfield condenser lens must be used, which directs a cone of light away from the objective lens. To maximize the scattered light-gathering power of the objective lens, oil immersion is used and the numerical aperture (NA) of the objective lens must be less than 1.0. Objective lenses with a higher NA can be used but ...

5. Objective (optics) - Wikipedia

   en.wikipedia.org/wiki/Objective_(optics)

   Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...

6. Raman microscope - Wikipedia

   en.wikipedia.org/wiki/Raman_microscope

   The sample is placed in the vacuum chamber of the electron microscope. Both analysis methods are then performed automatically at the same sample location. The obtained SEM and Raman images can then be superimposed. [20] [21] Moreover, adding a focused ion beam (FIB) on the chamber allows removal of the material and therefore 3D imaging of the ...

7. Total internal reflection fluorescence microscope - Wikipedia

   en.wikipedia.org/wiki/Total_internal_reflection...

   A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is supported on a glass slide.

8. Optical microscope - Wikipedia

   en.wikipedia.org/wiki/Optical_microscope

   Some microscopes make use of oil-immersion objectives or water-immersion objectives for greater resolution at high magnification. These are used with index-matching material such as immersion oil or water and a matched cover slip between the objective lens and the sample. The refractive index of the index-matching material is higher than air ...

9. Condenser (optics) - Wikipedia

   en.wikipedia.org/wiki/Condenser_(optics)

   As with objective lenses, a condenser lens with a maximum numerical aperture of greater than 0.95 is designed to be used under oil immersion (or, more rarely, under water immersion), with a layer of immersion oil placed in contact with both the slide/coverslip and the lens of the condenser. An oil immersion condenser may typically have NA of up ...