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Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2] This is called the stationary phase. [2]
The chiral stationary phase can be prepared by mixing chirally pure reagents such as L-amino acid, or brucine, or a chiral ligand exchange reagent with silica gel slurry, [2] [3] or by impregnation of the TLC plate in the solution of a chiral reagent. [4]
The spot capacity (analogous to peak capacity in HPLC) can be increased by developing the plate with two different solvents, using two-dimensional chromatography. [8] The procedure begins with development of a sample loaded plate with first solvent. After removing it, the plate is rotated 90° and developed with a second solvent.
Phosphomolybdic is used as a stain for developing thin-layer chromatography plates, [4] staining phenolics, hydrocarbon waxes, alkaloids, and steroids. Conjugated unsaturated compounds reduce PMA to molybdenum blue. The color intensifies with increasing number of double bonds in the molecule being stained. [5]
Served on a styrofoam plate with chips and a beer, it was humble, extremely satisfying, and darn delicious," says Josh Miller, Senior Food Editor. Read the original article on Southern Living.
Construction site safety is an aspect of construction-related activities concerned with protecting construction site workers and others from death, injury, disease or other health-related risks. Construction is an often hazardous, predominantly land-based activity where site workers may be exposed to various risks, some of which remain ...
STATE OF COLORADO Department of State 1700 Broadway Suite 200 Denver, CO 80290 Scott Gessler Secretary of State William A. Hobbs Deputy Secretary of State Main Number Administrati
Heavy machinery is seen Thursday at the site of a massive sinkhole that formed off Interstate 80 in New Jersey, just west of New York City, as crews evaluate the depression to design a repair.