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Bacterial growth curve\Kinetic Curve. In autecological studies, the growth of bacteria (or other microorganisms, as protozoa, microalgae or yeasts) in batch culture can be modeled with four different phases: lag phase (A), log phase or exponential phase (B), stationary phase (C), and death phase (D).
Figure 1: A bi-phasic bacterial growth curve.. A growth curve is an empirical model of the evolution of a quantity over time. Growth curves are widely used in biology for quantities such as population size or biomass (in population ecology and demography, for population growth analysis), individual body height or biomass (in physiology, for growth analysis of individuals).
Subculturing can also be used for growth curve calculations (ex. generation time) [2] and obtaining log-phase microorganisms for experiments (ex. Bacterial transformation). [3] Typically, subculture is from a culture of a certain volume into fresh growth medium of equal volume, this allows long-term maintenance of the cell line.
The method of enumeration [9] of surviving cells used by VCC is termed quantitative growth kinetics (QGK). It relates the kinetic time taken for the turbidity of a bacterial batch microbiological culture in a well of a 96-well microplate to reach a threshold difference in turbidity to a 10-fold dilution series of calibration growth curves.
Following the allotted time, the plate is removed and checked for bacterial growth. If the broth became cloudy or a layer of cells formed at the bottom, then bacterial growth has occurred. The results of the broth microdilution method are reported in Minimum Inhibitory Concentration (MIC), or the lowest concentration of antibiotics that stopped ...
The Monod equation is a mathematical model for the growth of microorganisms. It is named for Jacques Monod (1910–1976, a French biochemist, Nobel Prize in Physiology or Medicine in 1965), who proposed using an equation of this form to relate microbial growth rates in an aqueous environment to the concentration of a limiting nutrient.
The Atlanta Fed upgraded its gross domestic product growth estimate for the fourth quarter to a 3.2% annualized rate from a 2.7% pace earlier. The economy grew at a 2.8% rate in the third quarter.
A diauxic growth curve refers to the growth curve generated by an organism which has two growth peaks. The theory behind the diauxic growth curve stems from Jacques Monod's Ph.D. research in 1940. A simple example involves the bacterium Escherichia coli ( E. coli ), the best understood bacterium.