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These pollen grains were then germinated in vitro and GUS signals were observed within the tip growth of the pollen tubes. However, the strength of these GUS signals varied at different germination stages. The GUS signals were weak within the pollen tube tip at the early germination stage, but stronger at the later germination stages.
Germination is the process by which an organism grows from a seed or spore. The term is applied to the sprouting of a seedling from a seed of an angiosperm or gymnosperm, the growth of a sporeling from a spore, such as the spores of fungi, ferns, bacteria, and the growth of the pollen tube from the pollen grain of a seed plant.
Pollen germination has three stages; hydration, activation and pollen tube emergence. The pollen grain is severely dehydrated so that its mass is reduced, enabling it to be more easily transported from flower to flower. Germination only takes place after rehydration, ensuring that premature germination does not take place in the anther.
Pollen itself is not the male gamete. [4] It is a gametophyte, something that could be considered an entire organism, which then produces the male gamete.Each pollen grain contains vegetative (non-reproductive) cells (only a single cell in most flowering plants but several in other seed plants) and a generative (reproductive) cell.
The pollen is carried to the pistil of another flower, by wind or animal pollinators, and deposited on the stigma. As the pollen grain germinates, the tube cell produces the pollen tube, which elongates and extends down the long style of the carpel and into the ovary, where its sperm cells are released in the megagametophyte.
If the pollen is compatible it will germinate and begin to grow. [5] The ovary releases chemicals that stimulates a positive chemotropic response from the developing pollen tube. [6] In response the tube develops a defined tip growth area that promotes directional growth and elongation of the pollen tube due to a calcium gradient. [5]
Epilobium pollen has three apertures that are pores The aperture of Lilium pollen is a single sulcus. Apertures are areas on the walls of a pollen grain, where the wall is thinner and/or softer. For germination it is necessary that the pollen tube can reach out from the inside of the pollen grain and transport the sperm to the egg deep down in ...
The best studied mechanisms of SI act by inhibiting the germination of pollen on stigmas, or the elongation of the pollen tube in the styles. These mechanisms are based on protein-protein interactions, and the best-understood mechanisms are controlled by a single locus termed S, which has many different alleles in the species population.