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The first alkaline lysis was performed by Birnom and Doly in 1979. [1] Since then, slight modifications have been made to the procedure to get to today's most widely used approach. The steps of alkaline lysis can be summarized as the formation of a pellet, resuspension of the pellet in solution, cell lysis, neutralization, and centrifugation.
Cryoprecipitate, also called cryo for short, or Cryoprecipitate Antihemophilic factor (AHF), is a frozen blood product prepared from blood plasma. [1] To create cryoprecipitate, Plasma is slowly thawed to 1–6 °C. A cold-insoluble precipitate is formed, which is collected by centrifugation, resuspended in a small amount of residual plasma ...
A perturbed problem whose solution can be approximated on the whole problem domain, whether space or time, by a single asymptotic expansion has a regular perturbation.Most often in applications, an acceptable approximation to a regularly perturbed problem is found by simply replacing the small parameter by zero everywhere in the problem statement.
A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular biology experiments that analyze the labile macromolecules of the cells (e.g. western blot for protein, or for DNA extraction).
Hilbert's tenth problem: the problem of deciding whether a Diophantine equation (multivariable polynomial equation) has a solution in integers. Determining whether a given initial point with rational coordinates is periodic, or whether it lies in the basin of attraction of a given open set, in a piecewise-linear iterated map in two dimensions ...
A problem set, sometimes shortened as pset, [1] is a teaching tool used by many universities. Most courses in physics, math, engineering, chemistry, and computer science will give problem sets on a regular basis. [2] They can also appear in other subjects, such as economics.
Ribonuclease (commonly abbreviated RNase) is a type of nuclease that catalyzes the degradation of RNA into smaller components. Ribonucleases can be divided into endoribonucleases and exoribonucleases, and comprise several sub-classes within the EC 2.7 (for the phosphorolytic enzymes) and 3.1 (for the hydrolytic enzymes) classes of enzymes.
Typically, the size of the transported sediment is fine sand (<1 mm) and smaller, because air is a fluid with low density and viscosity, and can therefore not exert very much shear on its bed. Bedforms are generated by aeolian sediment transport in the terrestrial near-surface environment.