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Biophysical chemistry is a physical science that uses the concepts of physics and physical chemistry for the study of biological systems. [1] The most common feature of the research in this subject is to seek an explanation of the various phenomena in biological systems in terms of either the molecules that make up the system or the supra-molecular structure of these systems. [2]
Loop-mediated isothermal amplification (LAMP) primers [1] Loop-mediated isothermal amplification (LAMP) product [1]. In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C (140–149 °F) using either two or three sets of primers and a polymerase like Bst Klenow fragment with high strand displacement activity in addition to a replication activity.
A ribosome is a biological machine that utilizes protein dynamics Fluorescent imaging techniques, as well as electron microscopy , x-ray crystallography , NMR spectroscopy , atomic force microscopy (AFM) and small-angle scattering (SAS) both with X-rays and neutrons (SAXS/SANS) are often used to visualize structures of biological significance.
Physical biochemistry is a branch of biochemistry that deals with the theory, techniques, and methodology used to study the physical chemistry of biomolecules. [1] It also deals with the mathematical approaches for the analysis of biochemical reaction and the modelling of biological systems. It provides insight into the structure of ...
Circular Dichroism – method to measure chirality of a sample using circularly polarized light. This technique is commonly used to determine protein structure. Computational chemistry – use of numerical methods to probe the structure and dynamical equilibrium in biological systems. Cryobiology – studies the effects of low temperatures on ...
The methods in this section are primarily computational although they typically require data generated by wet lab experiments. Protein–protein docking , the prediction of protein–protein interactions based only on the three-dimensional protein structures from X-ray diffraction of protein crystals might not be satisfactory.
The Journal of Proteomics is a peer-reviewed scientific journal published by Elsevier. It is the official journal of the European Proteomics Association and the editor-in-chief is Juan Calvete. [1] It was established in 1979 as the Journal of Biochemical and Biophysical Methods, [2] obtaining its current name in 2008. [3]
Membrane biophysics is the study of biological membrane structure and function using physical, computational, mathematical, and biophysical methods. A combination of these methods can be used to create phase diagrams of different types of membranes, which yields information on thermodynamic behavior of a