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Frozen section procedure: water-rich tissues are hardened by freezing and cut in the frozen state with a freezing microtome or microtome-cryostat; sections are stained and examined with a light microscope. This technique is much faster than traditional histology (5 minutes vs 16 hours) and is used in conjunction with medical procedures to ...
Multi-tissue blocks were first introduced by H. Battifora in 1986 with his so-called “multitumor (sausage) tissue block" and modified in 1990 with its improvement, "the checkerboard tissue block" . In 1998, J. Kononen and collaborators developed the current technique, which uses a novel sampling approach to produce tissues of regular size and ...
The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic [3] Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after ...
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature (OCT) compound is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections) of a ...
Sectioning a tissue can use either the micro tome knife or the razor blade as the cutting blade. [4] The micro tome knife is used for handling sectioning. It is necessary to use a micro tome knife when preparing sections less than 1/1000 micrometers. [29] When using such a knife, the operators must be extremely careful.
Before selecting an area of the specimen block to be ultra-thin sectioned, the technician examines semithin or "thick" sections range from 0.5 to 2 μm. These thick sections are also known as survey sections and are viewed under a light microscope to determine whether the right area of the specimen is in a position for thin sectioning. "Ultra ...
The Mohs procedure is a pathology sectioning method that allows for the complete examination of the surgical margin. It is different from the standard bread loafing technique of sectioning, where random samples of the surgical margin are examined. [6]: 112–3 [2]: 3–4 [7] Mohs surgery is performed in four steps:
The Silicone S 10 is the procedure most often used in plastination and creates opaque, natural-looking specimen. [14] Dow Corning Corporation 's Cor-Tech Room Temperature Procedure is designed to allow plastination of specimen at room temperature to various degrees of flexibility using three combinations of polymer, crosslinker , and catalyst ...