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Classical patch clamp setup, with microscope, antivibration table, and micromanipulators. During a patch clamp recording, a hollow glass tube known as a micropipette or patch pipette filled with an electrolyte solution and a recording electrode connected to an amplifier is brought into contact with the membrane of an isolated cell.
A schematic of a patch clamp chip showing a gigaseal, whole cell recording configuration, and the ion channel and whole cell currents. Many types of systems have been developed for patch clamping cells in suspension cultures. One system uses a traditional pipette and cells in a droplet suspension culture to obtain patch clamp recordings (see ...
Patch-sequencing (patch-seq) is a modification of patch-clamp technique that combines electrophysiological, transcriptomic and morphological characterization of individual neurons. In this approach, the neuron's cytoplasm is collected and processed for RNAseq after electrophysiological recordings are performed on it.
The "patch-clamp" technique allows the study of individual ion channels. It uses an electrode with a relatively large tip (> 1 micrometer) that has a smooth surface (rather than a sharp tip). This is a "patch-clamp electrode" (as distinct from a "sharp electrode" used to impale cells).
Camtasia (/ k æ m ˈ t eɪ ʒ ə /; formerly Camtasia Studio [3] and Camtasia for Mac [4]) is a software suite, created and published by TechSmith, for creating and recording video tutorials and presentations via screencast (screen recording), or via a direct recording plug-in to Microsoft PowerPoint. Other multimedia recordings (microphone ...
This software is commonly used for desktop recording, gameplay recording and video editing. Screencasting software is typically limited to streaming and recording desktop activity alone, in contrast with a software vision mixer, which has the capacity to mix and switch the output between various input streams.
Video manipulation is a type of media manipulation that targets digital video using video processing and video editing techniques. The applications of these methods range from educational videos [ 1 ] to videos aimed at ( mass ) manipulation and propaganda , a straightforward extension of the long-standing possibilities of photo manipulation .
Many introductory video production courses cover the topic of in-camera editing for this very purpose. [ 4 ] The technique may also be used to limit directing and editing interference in a production (often on the part of producers or financiers) because the film exists only as shot, with no options for editing.