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Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose , a lactose metabolite that triggers transcription of the lac operon , and it is therefore used to induce protein expression where the gene is under the control of the lac operator .
IPTG is a reagent which mimics the structure of allolactose, and can therefore bind to the lac repressor and prevent it from inhibiting gene expression. Once enough IPTG is added, the T7 gene is normally transcribed and so transcription of the gene of interest downstream of the T7 promoter also begins. [6]
Once the cells reach a certain point in their growth, isopropyl β-D-1-thiogalactopyranoside (IPTG) is added. IPTG, a molecule similar to lactose, but with a sulfur bond that is not hydrolyzable so that E. coli does not digest it, is used to activate or "induce" the production of the new protein. Once the cells are induced, it is difficult to ...
A CPU cache is a piece of hardware that reduces access time to data in memory by keeping some part of the frequently used data of the main memory in a 'cache' of smaller and faster memory. The performance of a computer system depends on the performance of all individual units—which include execution units like integer, branch and floating ...
Adjust power settings for efficiency: Enhancing your computer's efficiency by adjusting power settings can extend battery life and improve performance. Optimize virtual memory and page files: This ...
It is thus similar to overhead in organizations. Computer system overhead shows up as slower processing, less memory, less storage capacity, less network bandwidth, or bigger latency than would be expected from reading the system specifications. [1] It is a special case of engineering overhead. Overhead can be a deciding factor in software ...
BERN (Reuters) - Switzerland said on Tuesday it was concerned by U.S. President-elect Donald Trump's proposals to raise tariffs and is considering how to respond if his new administration does so.
Average mortgage rates remain high across popular terms as of Friday, December 27, 2024, ending a quiet holiday week of consistent increases that have set borrowing costs for the 30-year benchmark ...