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Acetone–butanol–ethanol (ABE) fermentation, also known as the Weizmann process, is a process that uses bacterial fermentation to produce acetone, n-butanol, and ethanol from carbohydrates such as starch and glucose. It was developed by chemist Chaim Weizmann and was the primary process used to produce acetone, which was needed to make ...
The method has been described since as the ABE process, (Acetone Butanol Ethanol fermentation process), yielding 3 parts of acetone, 6 of n-butanol, and 1 of ethanol. Acetone was used in the important wartime task of casting cordite. The alcohols were used to produce vehicle fuels and synthetic rubber.
Fed-batch fermentation is a variation of batch fermentation where some of the ingredients are added during the fermentation. This allows greater control over the stages of the process. In particular, production of secondary metabolites can be increased by adding a limited quantity of nutrients during the non-exponential growth phase.
Acetone, butanol, and ethanol are the most common products of solventogenesis. Some species such as Clostridium beijerinckii, Clostridium puniceum and Clostridium roseum are able to further reduce acetone to isopropanol. Several species are able to produce additional solvents under various culture conditions.
Clostridium saccharobutylicum is an indole and notably acetone, butanol and ethanol-producing bacterium, with type strain DSM 13864 T (= ATCC BAA-117 T). [ 1 ] [ 2 ] Its genome has been sequenced. [ 3 ]
Ethanol is obtained by fermentation of glucose (which is often obtained from starch) in the presence of yeast. Carbon dioxide is cogenerated. Like ethanol, butanol can be produced by fermentation processes. Saccharomyces yeast are known to produce these higher alcohols at temperatures above 75 °F (24 °C).
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Perstraction has been combined with the ABE (acetone butanol ethanol) fermentation for butanol production. [1] Butanol is toxic to the fermentation, therefore perstraction can be applied to remove the butanol from the vicinity of the bacteria as soon as it is produced.