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Bacteria of the Legionella type (spread by contaminated mist) [3] [4] Risk factors: Older age, history of smoking, chronic lung disease, poor immune function [5] Diagnostic method: Urinary antigen test, sputum culture [6] Prevention: Good maintenance of water systems [7] Treatment: Antibiotics [8] Prognosis: 10% risk of death [8] Frequency
The tabled figures are for total aerobic plate count, cfu/ml at 30 °C (minimum 48 hours incubation) with colony count determined by the pour plate method according to ISO 6222(21) or spread plate method on yeast extract agar. Legionella isolation can be conducted using the method developed by the US Center for Disease Control using buffered ...
Legionella is a genus of gram-negative bacteria that can be seen using a silver stain or grown in a special media that contains cysteine, an amino acid.It is known to cause legionellosis [3] (all illnesses caused by Legionella) including a pneumonia-type illness called Legionnaires' disease and a mild flu-like illness called Pontiac fever. [3]
A consistent method that has been used to detect the disease is the urine antigen test. [7] Effective antibiotic treatment for Legionella pneumonia includes fluoroquinolones (levofloxacin or moxifloxacin) or, alternately, azithromycin.
Ionization can be an effective process to control Legionella in potable water distribution systems found in health facilities, hotels, nursing homes, and large buildings. . In 2003, ionization became the first such hospital disinfection process to have fulfilled a proposed four-step modality evaluation; by then it had been adopted by over 100 hospitals.
Legionella anisa is traditionally detected using the same culture methods as described for Legionella. However, some research suggests L. anisa may require a co-culture method that accounts for the close relationship with amoebae. When present in a sample but still contained within an amoeba host, the plating method may return false-negative ...
The dip slide test consists of a sterile culture medium on a plastic carrier that is dipped into the liquid to be sampled. [3] The culture is then incubated, allowing for microbial growth. [ 2 ] Most Dip slides consist of 1 - 2 agars attached to a flexible plastic paddle, this allows full contact of the agar onto the desired area for testing. [ 4 ]
Legionella testing typically involves collecting water samples and surface swabs from evaporative cooling basins, shower heads, faucets/taps, and other locations where warm water collects. The samples are then cultured and colony forming units (cfu) of Legionella are quantified as cfu/liter.
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