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The blue–white screen is a screening technique that allows for the rapid and convenient detection of recombinant bacteria in vector-based molecular cloning experiments. This method of screening is usually performed using a suitable bacterial strain , but other organisms such as yeast may also be used.
This allows for blue–white screening when using host strains such as E. coli JM109, which produces only the C-terminal portion of lacZ, also known as the β-polypeptide. [3] If pUC19 is inserted into E. coli JM109 and grown on agar media supplemented with IPTG and X-gal , then colonies will appear blue, as the plasmid encodes for the α ...
DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three [1] mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening.
This diagram was created with CorelDRAW. ... The following 2 pages use this file: Blue–white screen; ... some details may not fully reflect the modified file.
A selectable marker is a gene introduced into cells, especially bacteria or cells in culture, which confers one or more traits suitable for artificial selection.They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or transformation or other procedure meant to introduce foreign DNA into a cell.
By this method, body diagrams can be derived by pasting organs into one of the "plain" body images shown below. This method requires a graphics editor that can handle transparent images, in order to avoid white squares around the organs when pasting onto the body image. Pictures of organs are found on the project's main page. These were ...
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The [5 dl 2 dl 1 dl 2 dl] represents a dash-dot line. There are 5 units of line (the dash) followed by 2 units of empty space, 1 unit of line (the dot), 2 more units of empty space, and then it starts over again. 0.5 0.5 0.5 represents the color gray. /LTb is the graph's border, and /LTa is for the zero axes. [9]