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In vivo staining (also called vital staining or intravital staining) is the process of dyeing living tissues. By causing certain cells or structures to take on contrasting colours, their form or position within a cell or tissue can be readily seen and studied. The usual purpose is to reveal cytological details that might otherwise not be ...
Direct or substantive dyeing is normally carried out in a neutral or slightly alkaline dye bath, at or near boiling point, with the addition of either sodium chloride (NaCl) or sodium sulfate (Na 2 SO 4) or sodium carbonate (Na 2 CO 3). Direct dyes are used on cotton, paper, leather, wool, silk and nylon.
Also called direct dyes, substantive dyes are employed for cellulose-based textiles, which includes cotton. The dyes bind to the textile by non-electrostatic forces. In another classification, azo dyes can be classified according to the number of azo groups. Trypan blue is an example of a direct dye, used for cotton.
Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.
Direct blue 218 Direct blue 218 24401 diazo 28407-37-6: Direct brown 2 Direct brown 2 22311 diazo 2429-82-5: Direct sky blue 5B Direct sky blue FB Direct Blue 15: 22400 diazo 2429-74-5: Direct sky blue 6B Direct sky blue FF Direct Blue 1: 24410 diazo 2610-05-1: Direct yellow 4 Brilliant fast yellow Direct yellow 4 24890 diazo 3051-11-4: Direct ...
The common dyeing process of cotton yarn with reactive dyes at package form is as follows: The raw yarn is wound on a spring tube to achieve a package suitable for dye penetration. These softened packages are loaded on a dyeing carrier's spindle one on another. The packages are pressed up to a desired height to achieve suitable density of packing.
Substantive dyes work best on textiles with high contents of cellulose, such as cotton. In contrast to direct dyes, wool and leather goods are dyed by the process of ion exchange, exploiting the cationic nature of proteins near neutral pH. The development of substantive dyes helped make mordant dyes obsolete. [2]
Trypan blue is an azo dye. It is a direct dye for cotton textiles. [3] In biosciences, it is used as a vital stain to selectively colour dead tissues or cells blue. Live cells or tissues with intact cell membranes are not coloured. Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is ...