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Cresyl violet stained partial brain section of a Macaque. It is used in biology and medicine as a histological stain. Cresyl violet is an effective and reliable stain used for light microscopy sections. Initially, tissue sections are "defatted" by passing through graded dilutions of ethanol. Then, rehydrated by passing back through decreasing ...
Determining the viable cell count is important for calculating dilutions required for the passaging of cells, as well as determining the size and number of flasks needed during growth time. It is also vital when seeding plates for assays, such as the plaque assay , [ 2 ] because the plates need a known number of live replicating cells for the ...
The Gram-positive cell wall is characterized by the presence of a very thick peptidoglycan layer, which is responsible for the retention of the crystal violet dyes during the Gram staining procedure. It is found exclusively in organisms belonging to the Actinomycetota (or high %G+C Gram-positive organisms) and the Bacillota (or low %G+C Gram ...
In hemocytometry, Türk's solution (or Türk's fluid) is a hematological stain (either crystal violet or aqueous methylene blue) prepared in 99% acetic acid (glacial) [1] and distilled water. The solution destroys the red blood cells and platelets within a blood sample (acetic acid being the main lyzing agent ), and stains the nuclei of the ...
Cell counting is any of various methods for the counting or similar quantification of cells in the life sciences, including medical diagnosis and treatment. It is an important subset of cytometry , with applications in research and clinical practice.
The fixative is essential for good staining and presentation of cellular detail. After fixation, the slide is stained to distinguish the cells from each other. [citation needed] Routine analysis of blood in medical laboratories is usually performed on blood films stained with Romanowsky stains such as Wright's stain, Giemsa stain, or Diff-Quik ...
Gram-positive bacteria take up the crystal violet stain used in the test, and then appear to be purple-coloured when seen through an optical microscope. This is because the thick layer of peptidoglycan in the bacterial cell wall retains the stain after it is washed away from the rest of the sample, in the decolorization stage of the test.
Increasing amounts of cells resulted in increased purple colouring. The MTT assay is a colorimetric assay for assessing cell metabolic activity. [ 1 ] [ 2 ] NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present.