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The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added ...
Microtiter plates with 96, 384 and 1536 wells. A microplate, also known as a microtiter plate, microwell plate or multiwell, [1] is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories.
A microtiter plate after an MTT assay. Increasing amounts of cells resulted in increased purple colouring. The MTT assay is a colorimetric assay for assessing cell metabolic activity. [1] [2] NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present.
Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. They are widely used in research, drug discovery , [ 1 ] bioassay validation, quality control and manufacturing processes in the pharmaceutical and ...
A general procedure for HA is as follows, a serial dilution of virus is prepared across the rows in a U or V- bottom shaped 96-well microtiter plate. [5] The most concentrated sample in the first well is often diluted to be 1/5x of the stock, and subsequent wells are typically two-fold dilutions (1/10, 1/20, 1/40, etc.).
Cell lysates are collected and are serially diluted six to ten times if using colorimetric techniques, or without dilution when fluorometric detection is used (due to the greater dynamic range of fluorescence than colorimetric detection). Serial dilutions are then plated in replicates into a 384- or a 1536-well microtiter plate. [1]
During testing, multiple microtiter plates are filled with a certain broth, according to the needs of target bacteria. [3] Varying concentrations of the antibiotics and the bacteria to be tested are then added to the plate. The plate is then placed into a non-CO 2 incubator and incubated at thirty-seven degrees Celsius for sixteen to twenty ...
ELISA plate showing various cortisol levels Enzymes used in ELISAs include horseradish peroxidase (HRP), alkaline phosphatase (AP) or glucose oxidase . These enzymes allow for detection often because they produce an observable color change in the presence of certain reagents.