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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate , sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate .
Sodium dihydrogen phosphate Sodium hydrogen phosphate Trisodium phosphate. A sodium phosphate is a generic variety of salts of sodium (Na +) and phosphate (PO 3− 4). Phosphate also forms families or condensed anions including di-, tri-, tetra-, and polyphosphates. Most of these salts are known in both anhydrous (water-free) and hydrated forms ...
The use of Phospho soda has been known to lead to acute phosphate nephropathy. According to the U.S. Food and Drug Administration (FDA), "Acute phosphate nephropathy is a form of acute kidney injury that is associated with deposits of calcium-phosphate crystals in the renal tubules that may result in permanent renal function impairment.
RIPA buffer is a commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues. The buffer can be stored without vanadate at 4 °C for up to 1 year. [10] RIPA buffer releases proteins from cells as well as disrupts most weak interactions between proteins. [9] Recipe: [10] 1% (w/w) Nonidet P-40 (NP-40)
Sodium hexametaphosphate (SHMP) is a salt of composition Na 6 [(PO 3) 6]. [3] Sodium hexametaphosphate of commerce is typically a mixture of metaphosphates (empirical formula: NaPO 3), of which the hexamer is one, and is usually the compound referred to by this name. Such a mixture is more correctly termed sodium polymetaphosphate.
Sodium monofluorophosphate is produced industrially by the reaction of sodium fluoride with sodium metaphosphate: [2] NaPO 3 + NaF → Na 2 PO 3 F. The process involves scission of a pyrophosphate bond, analogous to hydrolysis. SMFP can also be prepared by treating tetrasodium pyrophosphate or disodium phosphate with hydrogen fluoride. [2]
Disodium phosphate (DSP), or disodium hydrogen phosphate, or sodium phosphate dibasic, is an inorganic compound with the chemical formula Na 2 H P O 4. It is one of several sodium phosphates. The salt is known in anhydrous form as well as hydrates Na 2 HPO 4 ·nH 2 O, where n is 2, 7, 8, and 12. All are water-soluble white powders. The ...
The majority of biological samples that are used in research are kept in a buffer solution, often phosphate buffered saline (PBS) at pH 7.4. In industry, buffering agents are used in fermentation processes and in setting the correct conditions for dyes used in colouring fabrics. They are also used in chemical analysis [5] and calibration of pH ...