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Flow cytometry gating into main categories of blood cells by side scatter and CD45. A gate in cytometry is a set of value limits (boundaries) that serve to isolate a specific group of cytometric events from a large set. Gates can be defined by discrimination analysis, or can simply be drawn around a given set of data points on a printout and ...
Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. [1] [2] [3] [4]In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.
Detection of fluorescent probe binding by the cells requires the use of flow cytometry preferably employing 6 to 8 different fluorescent probes that bind to different markers on 5 million cells from the patient's blood. The table also includes the percentage of MLB cases with the phenotype and the malignancies to which they progress.
Instead, plasma cells are identified through flow cytometry by their additional expression of CD138, CD78, and the Interleukin-6 receptor. In humans, CD27 is a good marker for plasma cells; naïve B cells are CD27−, memory B-cells are CD27+ and plasma cells are CD27++. [5] The surface antigen CD138 (syndecan-1) is expressed at high levels. [6]
Cell populations detected by flow cytometry are often described as having approximately log-normal expression. [15] As such, they have traditionally been transformed to a logarithmic scale. In early cytometers, this was often accomplished even before data acquisition by use of a log amplifier. On modern instruments, data is usually stored in ...
B cells, also known as B lymphocytes, are a type of white blood cell of the lymphocyte subtype. [1] They function in the humoral immunity component of the adaptive immune system. [1] B cells produce antibody molecules which may be either secreted or inserted into the plasma membrane where they serve as a part of B-cell receptors. [2]
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