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Illustration of streak plate procedure to achieve isolated colonies using aseptic technique. The three-phase streaking pattern, known as the T-Streak, is recommended for beginners. The streaking is done using a sterile tool, such as a cotton swab or commonly an inoculation loop. The inoculation loop is first sterilized by passing it through a ...
In the field of molecular biology, enterotoxin type B, also known as Staphylococcal enterotoxin B (SEB), is an enterotoxin produced by the gram-positive bacteria Staphylococcus aureus. It is a common cause of food poisoning , with severe diarrhea , nausea and intestinal cramping often starting within a few hours of ingestion. [ 1 ]
It has been suggested that staphylococci other than S. aureus can contribute to Staphylococcal Food Poisoning by forming enterotoxins. [6] Streptococcal exotoxins are produced by Streptococcus pyogenes. [7] [8] These toxins share the ability to bind to the major histocompatibility complex proteins of their hosts.
Streak a beta-lysin–producing strain of aureus down the center of a sheep blood agar plate. The test organism streak should be 3 to 4 cm long. Streak test organisms across the plate perpendicular to the S. aureus streak within 2 mm. (Multiple organisms can be tested on a single plate). Incubate at 35°-37°C in ambient air for 18-24 hours.
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate.
Industrial oil sold as food oil. [32] 1955: Morinaga milk arsenic poisoning [33] [34] powdered milk: arsenic: Japan: 13,389 >600: By mistake, an industrial grade Monosodium phosphate was added to milk produced by Morinaga Milk Industry, which contained an impurity of 5–8% arsenic. The milk powder was used for feeding infants, and many babies ...
Colonies of B. cereus were originally isolated by Percy F. Frankland from a gelatine plate left exposed to the air in a cow shed in 1887. [11] In the 2010s, examination of warning letters issued by the US Food and Drug Administration issued to pharmaceutical manufacturing facilities addressing facility microbial contamination revealed that the most common contaminant was B. cereus.
The 1996 Odwalla E. coli outbreak began on October 7, 1996, when American food company Odwalla produced a batch of unpasteurized apple juice using blemished fruit contaminated with the E. coli bacterium, which ultimately killed a 16-month-old girl and sickened 70 people in California, Colorado, Washington state, and British Columbia, of whom 25 were hospitalized and 14 developed hemolytic ...