Ads
related to: chip sequencing analysis- Epitranscriptomics
Single-base resolution Seq Services
5mC, 5hmC, m6A, m6Am, acetylation
- tRNA Sequencing
Highly Experienced
Gain insights into the expression
- Ribosome Profiling
Monitor Ribosome Activity
Simplify Your RNA Research
- Ultralow Input RNA-Seq
Low-cost & low-bias
Input amounts down to 2 pg
- Epitranscriptomics
Search results
Results from the WOW.Com Content Network
ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest.
ChIP has also been applied for genome-wide analysis by combining with microarray technology (ChIP-on-chip) or second-generation DNA-sequencing technology (Chip-Sequencing). ChIP can also combine with paired-end tags sequencing in Chromatin Interaction Analysis using Paired End Tag sequencing (ChIA-PET), a technique developed for large-scale, de ...
Introduced in 2007, ChIP sequencing (ChIP-seq) is a technology that uses chromatin immunoprecipitation to crosslink the proteins of interest to the DNA but then instead of using a micro-array, it uses the more accurate, higher throughput method of sequencing to localize interaction points. [13]
It is also possible to do more complex analysis using such tools like combining multiple ChIP-seq signal to detect regulatory sites. [10] In the context of ChIP-exo, this process is known as 'peak-pair calling'. [11] Differential peak calling is about identifying significant differences in two ChIP-seq signals. One can distinguish between one ...
ChIP-loop combines 3C with ChIP-seq to detect interactions between two loci of interest mediated by a protein of interest. [2] [46] The ChIP-loop may be useful in identifying long-range cis-interactions and trans interaction mediated through proteins since frequent DNA collisions will not occur. [citation needed]
Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET or ChIA-PETS) is a technique that incorporates chromatin immunoprecipitation (ChIP)-based enrichment, chromatin proximity ligation, Paired-End Tags, and High-throughput sequencing to determine de novo long-range chromatin interactions genome-wide.
Any platform that can allow for the ligated fragments to be sequenced across the NheI junction (Roche 454) or by paired-end or mate-paired reads (Illumina GA and HiSeq platforms) would be suitable for Hi-C. [4] Before high-throughput sequencing, the quality of the library should be verified using Sanger sequencing, wherein the long sequencing ...
Gene-set analysis (for example using tools like DAVID and GoSeq) has been shown to be severely biased when applied to high-throughput methylation data (e.g. MeDIP-seq and MeDIP-ChIP); it has been suggested that this can be corrected using sample label permutations or using a statistical model to control for differences in the numberes of CpG ...
Ads
related to: chip sequencing analysis