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Multicistronic message is an archaic term for Polycistronic. Monocistronic, bicistronic and tricistronic are also used to describe mRNA with single, double and triple coding areas (exons). Note that the base word cistron is no longer used in genetics, and has been replaced by intron and exon in eukaryotic mRNA. However, the mRNA found in ...
A cistron is a region of DNA that is conceptually equivalent to some definitions of a gene, such that the terms are synonymous from certain viewpoints, [1] especially with regard to the molecular gene as contrasted with the Mendelian gene.
An mRNA molecule is said to be monocistronic when it contains the genetic information to translate only a single protein chain (polypeptide). This is the case for most of the eukaryotic mRNAs. [ 28 ] [ 29 ] On the other hand, polycistronic mRNA carries several open reading frames (ORFs), each of which is translated into a polypeptide.
In the case of messenger RNA (mRNA) the RNA is an information carrier coding for the synthesis of one or more proteins. mRNA carrying a single protein sequence (common in eukaryotes) is monocistronic whilst mRNA carrying multiple protein sequences (common in prokaryotes) is known as polycistronic.
[63] [64] The genes in an operon are transcribed as a continuous messenger RNA, referred to as a polycistronic mRNA. The term cistron in this context is equivalent to gene. The transcription of an operon's mRNA is often controlled by a repressor that can occur in an active or inactive state depending on the presence of specific metabolites. [65]
A typical operon. In genetics, an operon is a functioning unit of DNA containing a cluster of genes under the control of a single promoter. [1] The genes are transcribed together into an mRNA strand and either translated together in the cytoplasm, or undergo splicing to create monocistronic mRNAs that are translated separately, i.e. several strands of mRNA that each encode a single gene product.
Monocistronic is a term used in biochemistry to describe the capacity of eukaryotes to code one gene per one mRNA, as opposed to prokaryotes which can code many genes, sometimes all, on one mRNA, polycistronic.
An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell. [1] In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s).