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Starting with the launch of the NextSeq and later the MiniSeq, Illumina introduced a new two-color sequencing chemistry. Nucleotides are distinguished by either one of two colors (red or green), no color ("black") or combining both colors (appearing orange as a mixture between red and green). Tagged nucleotides are added in order to the DNA strand.
Illumina rejected the offer, [17] and Roche abandoned the offer in April. [18] In 2014, the company announced a multimillion-dollar product, HiSeq X Ten. [19] [20] In January 2014, Illumina already held 70% of the market for genome-sequencing machines. [21] Illumina machines accounted for more than 90% of all DNA data produced. [22]
Library quality was first assessed on the Illumina MiSeq (2x84 np paired-end reads) platform and once passed quality control criteria (including low PCR duplicates), the library was sequenced on Illumina NextSeq (2x80 bp paired-end). [23]
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In 2010 Illumina released the HiSeq 2000 with an output of 200 and then 600G per run which would take 8 days. At its release the HiSeq 2000 provided one of the cheapest sequencing platforms at $0.02 per million bases as costed by the Beijing Genomics Institute. In 2011 Illumina released a benchtop sequencer called the MiSeq.
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An Illumina HiSeq 2500 sequencer Illumina NovaSeq 6000 flow cell. In this method, DNA molecules and primers are first attached on a slide or flow cell and amplified with polymerase so that local clonal DNA colonies, later coined "DNA clusters", are formed. To determine the sequence, four types of reversible terminator bases (RT-bases) are added ...
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