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In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Without oil, light waves reflect off the slide specimen ...
where θ is the angular resolution , λ is the wavelength of light, and D is the diameter of the lens' aperture. The factor 1.22 is derived from a calculation of the position of the first dark circular ring surrounding the central Airy disc of the diffraction pattern. This number is more precisely 1.21966989...
If the lens is held at a distance from the object such that its front focal point is on the object being viewed, the relaxed eye (focused to infinity) can view the image with angular magnification = Here, is the focal length of the lens in centimeters. The constant 25 cm is an estimate of the "near point" distance of the eye—the closest ...
The numerical aperture with respect to a point P depends on the half-angle, θ1, of the maximum cone of light that can enter or exit the lens and the ambient index of refraction. As a pencil of light goes through a flat plane of glass, its half-angle changes to θ2. Due to Snell's law, the numerical aperture remains the same: NA = n1 sin θ1 ...
Immersion lithography is a technique used in semiconductor manufacturing to enhance the resolution and accuracy of the lithographic process. It involves using a liquid medium, typically water, between the lens and the wafer during exposure. By using a liquid with a higher refractive index than air, immersion lithography allows for smaller ...
As with objective lenses, a condenser lens with a maximum numerical aperture of greater than 0.95 is designed to be used under oil immersion (or, more rarely, under water immersion), with a layer of immersion oil placed in contact with both the slide/coverslip and the lens of the condenser. An oil immersion condenser may typically have NA of up ...
In optical microscopes a darkfield condenser lens must be used, which directs a cone of light away from the objective lens. To maximize the scattered light-gathering power of the objective lens, oil immersion is used and the numerical aperture (NA) of the objective lens must be less than 1.0. Objective lenses with a higher NA can be used but ...
Angular field of view is typically specified in degrees, while linear field of view is a ratio of lengths. For example, binoculars with a 5.8 degree (angular) field of view might be advertised as having a (linear) field of view of 102 mm per meter. As long as the FOV is less than about 10 degrees or so, the following approximation formulas ...