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The '3T3' designation refers to the abbreviation of "3-day transfer, inoculum 3 × 105 cells." This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days ...
Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector. [ 1] An example is the viral transfer of DNA from one bacterium to another and hence an example of horizontal gene transfer. [ 2] Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA ...
Decoupage or découpage ( / ˌdeɪkuːˈpɑːʒ /; [ 1] French: [dekupaʒ]) is the art of decorating an object by gluing colored paper cutouts onto it in combination with special paint effects, gold leaf, and other decorative elements. Commonly, an object like a small box or an item of furniture is covered by cutouts from magazines or from ...
Bacterial conjugation. Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. [ 1] This takes place through a pilus. [ 2][full citation needed] It is a parasexual mode of reproduction in bacteria. A micrograph displaying Escherichia coli ...
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The central dogma of molecular biology deals with the flow of genetic information within a biological system. It is often stated as "DNA makes RNA, and RNA makes protein", [ 1] although this is not its original meaning. It was first stated by Francis Crick in 1957, [ 2][ 3] then published in 1958: [ 4][ 5]
Of these, the most commonly used vectors are plasmids. [2] Common to all engineered vectors are an origin of replication, a multicloning site, and a selectable marker. The vector itself generally carries a DNA sequence that consists of an insert (in this case the transgene) and a larger sequence that serves as the "backbone" of the vector. The ...
Chicago P.D. begins a month following his transfer. Aside from the undercover assignment that served as a premise for his transfer to Intelligence, little is known about his early CPD career, except that he was already friends with Antonio Dawson prior to the transfer and that he joined after serving in the military.