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The '3T3' designation refers to the abbreviation of "3-day transfer, inoculum 3 × 105 cells." This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days ...
Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector. [ 1] An example is the viral transfer of DNA from one bacterium to another and hence an example of horizontal gene transfer. [ 2] Transduction does not require physical contact between the cell donating the DNA and the cell receiving the DNA ...
Horizontal gene transfer ( HGT) or lateral gene transfer ( LGT) [ 1][ 2][ 3] is the movement of genetic material between organisms other than by the ("vertical") transmission of DNA from parent to offspring ( reproduction ). [ 4] HGT is an important factor in the evolution of many organisms. [ 5][ 6] HGT is influencing scientific understanding ...
Decoupage or découpage ( / ˌdeɪkuːˈpɑːʒ /; [ 1] French: [dekupaʒ]) is the art of decorating an object by gluing colored paper cutouts onto it in combination with special paint effects, gold leaf, and other decorative elements. Commonly, an object like a small box or an item of furniture is covered by cutouts from magazines or from ...
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Bacterial conjugation. Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. [ 1] This takes place through a pilus. [ 2][full citation needed] It is a parasexual mode of reproduction in bacteria. A micrograph displaying Escherichia coli ...
The central dogma of molecular biology deals with the flow of genetic information within a biological system. It is often stated as "DNA makes RNA, and RNA makes protein", [ 1] although this is not its original meaning. It was first stated by Francis Crick in 1957, [ 2][ 3] then published in 1958: [ 4][ 5]
Of these, the most commonly used vectors are plasmids. [2] Common to all engineered vectors are an origin of replication, a multicloning site, and a selectable marker. The vector itself generally carries a DNA sequence that consists of an insert (in this case the transgene) and a larger sequence that serves as the "backbone" of the vector. The ...