Search results
Results from the WOW.Com Content Network
In microbiology, in vivo is often used to refer to experimentation done in a whole organism, rather than in live isolated cells, for example, cultured cells derived from biopsies. In this situation, the more specific term is ex vivo. Once cells are disrupted and individual parts are tested or analyzed, this is known as in vitro. [citation needed]
Building a consistent and reliable extrapolation procedure from in vitro results to in vivo is therefore extremely important. Solutions include: Increasing the complexity of in vitro systems to reproduce tissues and interactions between them (as in "human on chip" systems) [40]
The problem of transposing in vitro results is particularly acute in areas such as toxicology where animal experiments are being phased out and are increasingly being replaced by alternative tests. Results obtained from in vitro experiments cannot often be directly applied to predict biological responses of organisms to chemical exposure in vivo.
The term ex vivo means that the samples to be tested have been extracted from the organism. The term in vitro (lit. "within the glass") means the samples to be tested are obtained from a repository. In the case of cancer cells, a strain that would produce favorable results, then grown to produce a control sample and the number of samples ...
In vitro muscle testing is a method used to characterize properties of living muscle tissue after removing it from an organism, which allows more extensive and precise quantification of its properties than in vivo testing. In vitro muscle testing has provided the bulk of scientific knowledge of muscle structure and physiology, and how both ...
Serial passage can either be performed in vitro or in vivo. In the in vitro method, a virus or a strain of bacteria will be isolated and allowed to grow for a certain time. After the sample has grown for that time, part of it will be transferred to a new environment and allowed to grow for the same period.
In 1949, the Milas hydroxylation was applied to a study of the demethylation of N-Dimethyl-p-Amino-azobenzene, otherwise known as butter yellow. Hydrogen peroxide in tertiary butyl alcohol with osmium tetroxide as a catalyst (Milas reagents) was examined to determine the parallels of the reaction with butter yellow in vivo versus in vitro ...
A 3D cell culture is an artificially created environment in which biological cells are permitted to grow or interact with their surroundings in all three dimensions. Unlike 2D environments (e.g. a Petri dish), a 3D cell culture allows cells in vitro to grow in all directions, similar to how they would in vivo. [1]