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The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. [ 1 ] The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber.
Until the 1950s the hemocytometer was the standard method to count blood cells. [5] In blood cell counting applications the hemocytometer has now been replaced by electronic cell counters. However, the hemocytometer is still being used to count cells in cell culture laboratories. Successively the manual task of counting, using a microscope, is ...
A complete blood count (CBC), also known as a full blood count (FBC), is a set of medical laboratory tests that provide information about the cells in a person's blood.The CBC indicates the counts of white blood cells, red blood cells and platelets, the concentration of hemoglobin, and the hematocrit (the volume percentage of red blood cells).
By the counting of cells in a known small volume, the concentration can be mediated. Examples of the need for cell counting include: In medicine, the concentration of various blood cells, such as red blood cells and white blood cells, can give crucial information regarding the health situation of a person (see: complete blood count). In cell ...
Instrument Uses Flow cytometer: used for automated cell counting as in total blood count, differential count, etc. : Tissue bath or organ bath or Dale's apparatus: used in full tissue experiments, for example using guinea pig ileum mainly used in pharmacology for application of drugs to these tissues.
Hematology analyzers are used to conduct a complete blood count (CBC), which is usually the first test requested by physicians to determine a patient's general health status. [5] A complete blood count includes red blood cell (RBC), white blood cell (WBC), hemoglobin, and platelet counts, as well as hematocrit levels.
A dilution of the cells to be counted is prepared and mixed with Trypan blue, this is normally the stain of choice because it is taken up by dead cells and actively excluded from live cells. Once the cells have been stained, they are counted using a hemocytometer, then a calculation is carried out to the original concentration of live cells. [1]
The hematocrit (/ h ɪ ˈ m æ t ə k r ɪ t /) (Ht or HCT), also known by several other names, is the volume percentage (vol%) of red blood cells (RBCs) in blood, [1] [2] measured as part of a blood test. [3] The measurement depends on the number and size of red blood cells. [3] It is normally 40.7–50.3% for males and 36.1–44.3% for ...