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TSI agar slant results: (from left) preinoculated (as control), P. aeruginosa, E. coli, Salmonella Typhimurium, Shigella flexneri The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. [1]
A TSI slant is often used to distinguish nonfermenting Pseudomonas species from enteric pathogens in faecal specimens. [ citation needed ] When P. aeruginosa is isolated from a normally sterile site (blood, bone, deep collections), it is generally considered dangerous, and almost always requires treatment.
Inoculation of a TSI slant shows an alkaline slant and acidic, but with no gas, or H 2 S production. Following incubation on SIM, the culture appears nonmotile with no H 2 S production. Addition of Kovac's reagent to the SIM tube following growth typically indicates no indole formation (serotypes 2, 7, and 8 produce indole [5]).
Simmons’ agar can be bought from suppliers as ready-made powders or slants. A slant is prepared by adding the heated agar to a test tube and allowing it to solidify at a slanted angle. To transfer cells from a sample to the agar, a sterilized needle is used to select a distinct colony from the sample and to streak across the agar surface, as ...
Pseudomonas infection refers to a disease caused by one of the species of the genus Pseudomonas. P. aeruginosa is a germ found in the environment and it is an opportunistic human pathogen most commonly infecting immunocompromised patients, such as those with cancer, diabetes, cystic fibrosis, [1] severe burns, AIDS, [2] or people who are very ...
The triple sugar iron (TSI) test is a differential media used to tell whether an organism can ferment glucose, sucrose, and/or lactose and whether an organism can produce hydrogen sulfide gas. [ 36 ] Urea agar slant
More mysterious symptoms started showing up—and I finally hit my breaking point. After about three months, I got a rash all over my legs. I had horrible cramping in my stomach, was exhausted all ...
Comparison of two culture media types used to grow Neisseria gonorrhoeae bacteria. Known as overgrowth, note that the non-selective chocolate agar medium on the left, due to its composition, allowed for the growth of organismal colonies other than those of Neisseria gonorrhoeae, while the selective Thayer–Martin medium on the right, containing antimicrobials that inhibit the growth of ...