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"YKL074C" denotes the 74th ORF to the left of the centromere of chromosome XI and that the coding strand is the Crick strand (C). Another confusing term referring to "Plus" and "Minus" strand is also widely used. Whether the strand is sense (positive) or antisense (negative), the default query sequence in NCBI BLAST alignment is "Plus" strand.
The minus-strand DNA is complete and spans the entire genome, while the plus strand spans only about two-thirds of the genome length and have variable 3' ends. But for the avihepadnaviruses they normally extend plus-strands almost all the way to the modified 5' end. [1]
Positive-strand RNA virus genomes usually contain relatively few genes, usually between three and ten, including an RNA-dependent RNA polymerase. [4] Coronaviruses have the largest known RNA genomes, between 27 and 32 kilobases in length, and likely possess replication proofreading mechanisms in the form of an exoribonuclease within nonstructural protein nsp14.
Negative-strand RNA viruses (−ssRNA viruses) are a group of related viruses that have negative-sense, single-stranded genomes made of ribonucleic acid (RNA). They have genomes that act as complementary strands from which messenger RNA (mRNA) is synthesized by the viral enzyme RNA-dependent RNA polymerase (RdRp). During replication of the ...
As a summary, a typical DNA rolling circle replication has five steps: [2] Circular dsDNA will be "nicked". The 3' end is elongated using "unnicked" DNA as leading strand (template); 5' end is displaced. Displaced DNA is a lagging strand and is made double stranded via a series of Okazaki fragments. Replication of both "unnicked" and displaced ...
The intracellular predomination of the long minus strand of DNA over the short plus strand suggests DNA replication proceeds from an RNA template, like other hepadnaviruses. [6] It is predicted that synthesis of the minus strand utilizes an RNA intermediate that is degraded as it is copied, and the plus strand is synthesized using the complete ...
By convention, the coding strand is the strand used when displaying a DNA sequence. It is presented in the 5' to 3' direction. Wherever a gene exists on a DNA molecule, one strand is the coding strand (or sense strand), and the other is the noncoding strand (also called the antisense strand, [3] anticoding strand, template strand or transcribed ...
The newly synthesized minus-strand cDNA copy of the 5'LTR is then transferred to the 3'LTR and used as a primer for reverse-transcription of the entire minus-strand sequence. An RNase H-resistant polypurine tract then serves as a primer for plus-strand synthesis of the 3'LTR and complementary PBS.