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Methylene blue is given as a 1% solution (10 mg/ml) 1 to 2 mg/kg administered intravenously slowly over five minutes. Although the response is usually rapid, the dose may be repeated in one hour if the level of methemoglobin is still high one hour after the initial infusion. Methylene blue inhibits monoamine oxidase, and serotonin toxicity can ...
Methylene blue has been described as "the first fully synthetic drug used in medicine". Methylene blue was first prepared in 1876 by German chemist Heinrich Caro. [64] Its use in the treatment of malaria was pioneered by Paul Guttmann and Paul Ehrlich in 1891. During this period before the first World War, researchers like Ehrlich believed that ...
The aqueous solution in the classical reaction contains glucose, sodium hydroxide and methylene blue. [14] In the first step an acyloin of glucose is formed. The next step is a redox reaction of the acyloin with methylene blue in which the glucose is oxidized to diketone in alkaline solution [6] and methylene blue is reduced to colorless leucomethylene blue.
A methylene blue active substances assay, or MBAS assay, is a colorimetric analysis test method that uses methylene blue to detect the presence of anionic surfactants (such as a detergent or foaming agent) in a sample of water. An anionic surfactant detected by the color reaction is called a methylene blue active substance (MBAS). [1]
A Ziehl–Neelsen stain is an acid-fast stain used to stain species of Mycobacterium tuberculosis that do not stain with the standard laboratory staining procedures such as Gram staining. This stain is performed through the use of both red coloured carbol fuchsin that stains the bacteria and a counter stain such as methylene blue.
(Vital stains include dyes like trypan blue and propidium iodide, which are either too bulky or too charged to cross the cell membrane, or which are actively rapidly pumped out by live cells.) Supravital staining can be combined with cell surface antibody staining ( immunofluorescence ) for applications such as FACS analysis. [ 4 ]
It is advised to check the references for photos of reaction results. [1] Reagent testers might show the colour of the desired substance while not showing a different colour for a more dangerous additive. [ 2 ]
It is used to stain collagen. If blue is preferred to green, methyl blue or water blue can be substituted. Standard applications: Masson's trichrome staining is widely used to study muscular pathologies (muscular dystrophy), cardiac pathologies , hepatic pathologies or kidney pathologies (glomerular fibrosis). It can also be used to detect and ...
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