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T4 is capable of undergoing only a lytic life cycle and not the lysogenic life cycle. The species was formerly named T-even bacteriophage, a name which also encompasses, among other strains (or isolates), Enterobacteria phage T2, Enterobacteria phage T4 and Enterobacteria phage T6.
Structural model at atomic resolution of bacteriophage T4 [1] The structure of a typical myovirus bacteriophage Anatomy and infection cycle of bacteriophage T4.. A bacteriophage (/ b æ k ˈ t ɪər i oʊ f eɪ dʒ /), also known informally as a phage (/ ˈ f eɪ dʒ /), is a virus that infects and replicates within bacteria and archaea.
In the lytic cycle, the viral DNA exists as a separate free floating molecule within the bacterial cell, and replicates separately from the host bacterial DNA, whereas in the lysogenic cycle, the viral DNA is integrated into the host genome. This is the key difference between the lytic and lysogenic cycles.
The prokaryotic cell is shown with its DNA, in green. 2. The bacteriophage attaches and releases its DNA, shown in red, into the prokaryotic cell. 3. The phage DNA then moves through the cell to the host's DNA. 4. The phage DNA integrates itself into the host cell's DNA, creating prophage. 5. The prophage then remains dormant until the host ...
The binding process is known as adsorption. [5] Once a phage adsorbs to the surface of a bacteria, it may undergo either the lytic cycle or the lysogenic cycle. [6] Virulent phages enter the lytic cycle where they replicate and lyse the bacterial cell. [7]
The selection and amplification steps can be performed multiple times at greater stringency to isolate higher-affinity binders. Phage display is a laboratory technique for the study of protein–protein , protein – peptide , and protein– DNA interactions that uses bacteriophages ( viruses that infect bacteria ) to connect proteins with the ...
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Bacteriophage T4 is a virus that infects the bacterium E. coli. Bacteriophage T4 genes are conventionally classified as early function genes or late function genes based on the time period in which their protein products are expressed during the course of bacteriophage infection.